Human DSC1 / Desmocollin 1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein A purified

Unmodified

Synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC). Percent identity by BLAST analysis: Human, Gibbon, Monkey (96%); Gorilla (92%); Marmoset (88%).

Intracellular

Antigen recognized in species specific for human. Cell Lines (tested so far) of differentiated cells. All simple epithelia are negative. desmocollin 1; negative with bovine (tested so far) and rodent material. Reacts with 111kD (form 'a') and 103kD (form 'b') polypeptides (desmocollin1 splice forms, both at pI 5.2) from human epidermal desmosomes. Reactivities on Cultured Positive on primary keratinocytes, however, restricted to local piles.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot

Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry (Frozen, Paraffin, HEIR): 1:10 in PBS, pH 7.4; Incubation Time 1 hr at RT or overnight at 4?C. Note: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended (see ref. 2). Without detergent pretreatment a cytoplasmic component might cross-react (e. g. in fibroblasts). (Paraffin: slight cross-reactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step. Localization: Localizes Dsc1 present in suprabasal layers of interfollicular epidermis; specific cell layers (e. g. in hair follicle root sheath); Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

Lyophilized PBS, pH 7.4, 0.09% Sodium Azide, 0.5% BSA

Reconstitute with 1.0 ml sterile ddH2O

Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q08554 NCBI: NM_024421 NP_077739.1