Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Rat DRD5 / Dopamine Receptor D5
IHC, Western blot
Rat DRD5 / Dopamine Receptor D5
Rat, Mouse (tested or 100% immunogen sequence identity)
Western blot (1:250 - 1:500)
Specificity and Use
DRD5 / Dopamine Receptor D5 antibody was raised against recombinant rat D1b Receptor, carboxy terminus
Dopamine Receptor D1b (D5)
Western blot: 1:250-1:500 on mouse brain lysate (unboiled sample). Immunohistochemistry. SUGGESTED WESTERN BLOT PROTOCOL. Solutions.10X Transfer buffer 1 liter. Tris 115.3 gm. Glycine 24.2 gm. [SDS 4 gm (may be omitted)]. For a 1X working solution: 1 lite
0.02 M phosphate buffer, pH 7.6, 0.25 M sodium chloride, 0.1% sodium azide
DRD5 / Dopamine Receptor D5 is the D5 subtype of the dopamine receptor. The D5 subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. This receptor is expressed in neurons in the limbic regions of the brain. It has a 10-fold higher affinity for dopamine than the D1 subtype. Pseudogenes related to this gene reside on chromosomes 1 and 2.