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Anti-DPP2 / DPP7 Antibody LS-C64891

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DPP2 / DPP7 Antibodies

Anti-DPP2 / DPP7 Antibody (Extracellular Domain) IHC-plus™ LS-A8015
Rabbit Polyclonal to Human DPP2 / DPP7
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-DPP2 / DPP7 Antibody (Extracellular Domain) IHC-plus™ LS-A8016
Rabbit Polyclonal to Human DPP2 / DPP7
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-DPP2 / DPP7 Antibody (Extracellular Domain) IHC-plus™ LS-A8018
Rabbit Polyclonal to Human DPP2 / DPP7
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal (IgG2a) to Human DPP2 / DPP7
Human
Western blot, ELISA, Neutralization
Unconjugated

Details

Human DPP2 / DPP7
Rat
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1 - 2 µg/ml)
  • ELISA
  • Neutralization

Specificity and Use

DPP2 / DPP7 antibody was raised against nS0-derived recombinant human rhDPPII.
Recognizes human DPPII. Species sequence homology: chimpanzee 99%, mouse 81%, rat 80%, dog 79% and chicken 65%.
Suitable for use in ELISA, Neutralization and Western blot. Neutralization-preincubated with the. enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then. assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by. the antibody at approximately 0.12 ug/ml (IC50) under conditions in which the enzyme was. present at 0.1 ug/ml and the substrate concentration was 10uM. Considering the molecular. masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at. approximately 1:2 molar ratio of the antibody to the enzyme. Western blot: 1-2 ug/ml The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with. WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.

Packaging

Lyophilized from PBS, 5% trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/ml.
100 µl PBS
For research use only.

About DPP2 / DPP7

Q9UHL4 NM_013379 NP_037511.2

DPP7 Antibody, Carboxytripeptidase Antibody, Dipeptidyl aminopeptidase II Antibody, Dipeptidyl peptidase 2 Antibody, DPPII Antibody, Dipeptidyl arylamidase II Antibody, Dipeptidyl-peptidase 7 Antibody, Dipeptidyl-peptidase II Antibody, Dipeptidylpeptidase 7 Antibody, Dipeptidyl peptidase 7 Antibody, Dipeptidyl peptidase II Antibody, DPP II Antibody, DPP2 Antibody, QPP Antibody

DPP2 / DPP7 is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH.

Requested From: United States
Date Requested: 12/7/2016

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