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DONSON / B17 Antibody (aa301‑350) LS‑C80782 Best Seller
B17 antibody LS-C80782 is an unconjugated rabbit polyclonal antibody to B17 (DONSON) from human, mouse, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C80782-50
50 µg
Unavailable

Popular DONSON / B17 Products

Western blot of DONSON antibody in HepG2 cell line lysates (35 ug/lane). DONSON (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Western blot of DONSON Antibody in HeLa cell line lysates (35 ug/lane). DONSON (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot, Flow Cytometry
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Mouse, Guinea pig, Horse, Rabbit
Applications: Western blot

Product Description

B17 antibody LS-C80782 is an unconjugated rabbit polyclonal antibody to B17 (DONSON) from human, mouse, guinea pig and other species. Validated for WB.
About DONSON / B17
Essential for DNA amplification in the ovary and required for cell proliferation during development. Q9NYP3 NM_017613 NP_060083.1

DONSON Antibody, B17 Antibody, C2TA Antibody, DKFZP434M035 Antibody, C21orf60 Antibody, Downstream neighbor of SON Antibody


Specifications

Target
Human DONSON / B17
Host
Rabbit
Reactivity
Human, Mouse, Guinea pig, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Bat, Bovine, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
DONSON / B17 antibody was raised against synthetic peptide located between aa301-350 of human DONSON (Q9NYP3, NP_060083). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Mouse, Rabbit, Horse, Guinea pig, Lizard (100%); Marmoset, Rat, Elephant, Dog, Bovine, Bat, Turkey, Stickleback (92%); Opossum, Chicken, Armadillo, Platypus, Xenopus (85%).
Blocking Peptide
LS-E16730 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80782. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa301-350
Specificity
Human DONSON
Usage
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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