B17 antibody LS-C80782 is an unconjugated rabbit polyclonal antibody to B17 (DONSON) from human, mouse, guinea pig and other species. Validated for WB.
Human, Mouse, Guinea pig, Horse, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
DONSON / B17 antibody was raised against synthetic peptide located between aa301-350 of human DONSON (Q9NYP3, NP_060083). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Mouse, Rabbit, Horse, Guinea pig, Lizard (100%); Marmoset, Rat, Elephant, Dog, Bovine, Bat, Turkey, Stickleback (92%); Opossum, Chicken, Armadillo, Platypus, Xenopus (85%).
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.