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Anti-DOK2 Antibody (C-Terminus) IHC-plus™ LS-B1520

Note: This antibody replaces LS-C30164

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DOK2 Antibodies

Anti-DOK2 Antibody (C-Terminus) IHC-plus™ LS-B1520
Rabbit Polyclonal (IgG) to Human DOK2
Human, Bovine, Guinea pig, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DOK2 Antibody (C-Terminus) LS-C50493
Rabbit Polyclonal to Human DOK2
Human
IHC
Unconjugated
Immunohistochemistry Image
Anti-DOK2 Antibody (clone 2D10) LS-C337459
Mouse Monoclonal [clone 2D10] (IgG1) to Human DOK2
Human, Monkey, Mouse, Rat, Dog
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human DOK2
Human, Bovine, Guinea pig, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human DOK2
Rabbit
Human, Bovine, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bat, Dog, Horse (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

DOK2 antibody was raised against synthetic peptide from C-Terminus of human DOK2 (O60496, NP_003965). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Bovine, Pig, Guinea pig (100%); Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse (90%).
LS-E10576 - Lyophilized - 100 µg - $145.00
C-Terminus
Human DOK2
Immunohistochemistry: LS-B1520 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1520 was determined to be 5 ug/ml.

Packaging

Lyophilized.
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About DOK2

O60496 NM_003974 NP_003965.2

DOK2 Antibody, Docking protein 2, 56kD Antibody, Dok-2 Antibody, Docking protein 2 Antibody, Docking protein 2, 56kDa Antibody, p56(dok-2) Antibody, p56dok-2 Antibody, p56DOK Antibody

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.

Immunohistochemistry

Immunohistochemistry
Anti-DOK2 antibody IHC of human liver, Kupffer cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1520 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
DOK2 antibody LS-B1520 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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