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Anti-DNMT3L Antibody (aa152-164) LS-C19023

Note: This antibody replaces LS-C64796

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DNMT3L Antibodies

Anti-DNMT3L Antibody (aa322-337) IHC-plus™ LS-B801
Rabbit Polyclonal to Human DNMT3L
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Immunohistochemistry Image
Anti-DNMT3L Antibody (aa357-388) LS-C96556
Rabbit Polyclonal to Human DNMT3L
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IHC - Paraffin, Western blot
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Anti-DNMT3L Antibody (aa389-421) LS-C96597
Rabbit Polyclonal to Human DNMT3L
Human, Mouse
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Western blot Image

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Rabbit Polyclonal to Human DNMT3L
Human
Western blot, ELISA
Unconjugated

Details

Human DNMT3L
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

DNMT3L antibody was raised against synthetic peptide from human DNMT3L.
aa152-164
aa 152-164 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About DNMT3L

Q9UJW3 NM_013369 NP_037501.2

DNMT3L Antibody

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity.

Western blot

Western blot
Anti-DNMT3L Antibody - Western Blot. Affinity Purified Rabbit anti-DNMT3L was used at a 1:1500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4 ug/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for western blotting only following immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected molecular weight of human DNMT3L is 43.6 kD.
 

Requested From: United States
Date Requested: 12/6/2016

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