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Antibodies > DNMT3B > LS-C352124
 
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Catalog Number Size Price
LS-C352124-100 100 µl (1 mg/ml) $299 
DNMT3B Antibody - Immunohistochemical analysis of DNMT3B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DNMT3B Antibody - Immunofluorescent analysis of DNMT3B staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DNMT3B Antibody - Western blot analysis of DNMT3B expression in A549 (A); COS7 (B); HeLa (C) whole cell lysates.
DNMT3B Antibody - Immunohistochemical analysis of DNMT3B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DNMT3B Antibody - Immunofluorescent analysis of DNMT3B staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DNMT3B Antibody - Western blot analysis of DNMT3B expression in A549 (A); COS7 (B); HeLa (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human DNMT3B Antibody (N‑Terminus, IHC, IF, WB) LS‑C352124

Polyclonal Rabbit anti‑Human DNMT3B Antibody (N‑Terminus, IHC, IF, WB) LS‑C352124

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
DNMT3B Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352124-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DNMT3B Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
DNMT3B antibody LS-C352124 is an unconjugated rabbit polyclonal antibody to DNMT3B (N-Terminus) from human. It is reactive with human and monkey. Validated for ICC, IF, IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human DNMT3B
Synonyms
DNMT3B | DNA MTase HsaIIIB | ICF | ICF1 | M.HsaIIIB | DNA methyltransferase HsaIIIB
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human DNMT3B.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of DNMT3B protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DNMT3B
Q9UBC3 NM_006892 NP_008823.1

LSBio Ratings

DNMT3B Antibody (N-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C352124 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Epigenetic Dysregulation of Trophoblastic Gene Expression in Gestational Trophoblastic Disease. Zoltan Szabolcsi, Amanda Demeter, Peter Kiraly, Andrea Balogh, Melissa L Wilson, Jennifer R King, Szabolcs Hetey, Zsolt Gelencser, Koji Matsuo, Beata Hargitai, Paulette Mhawech-Fauceglia, Petronella Hupuczi, Andras Szilagyi, Zoltan Papp, Lynda D Roman, Victoria K Cortessis, Nandor Gabor Than. Biomedicines. 2021 December;9:
Full Text Article PubMed: 34944751 PMC: PMC8698431
Submit a Publication for LS-C352124

Customer Reviews (0)

Submit a review for LS-C352124

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
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