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Anti-DNMT / DNMT1 Antibody (aa151-200) IHC-plus™ LS-B5632

Note: This antibody replaces LS-C116039

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450

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DNMT / DNMT1 Antibody LS-B5632 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-DNMT1 antibody IHC of mouse lymphoid tissue.
Western blot
DNMT1 antibody LS-B5632 Western blot of Mouse Lung lysate.

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Rabbit Polyclonal to Mouse DNMT / DNMT1
Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Mouse DNMT / DNMT1
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.2 - 1 µg/ml)

Specificity and Use

DNMT / DNMT1 antibody was raised against synthetic peptide located between aa151-200 of mouse Dnmt1 (P13864, NP_034196). Percent identity by BLAST analysis: Mouse (100%).
LS-E13287 - Lyophilized - 100 µg - $145.00
aa151-200
Mouse DNMT1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About DNMT / DNMT1

P26358 NM_001379 NP_001370.1

DNMT1 Antibody, AIM Antibody, CXXC finger protein 9 Antibody, CXXC9 Antibody, DNA methyltransferase 1 Antibody, DNMT Antibody, DNA methyltransferase HsaI Antibody, DNA MTase HsaI Antibody, HSN1E Antibody, M.HsaI Antibody, MCMT Antibody

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones.

Immunohistochemistry

Immunohistochemistry
Anti-DNMT1 antibody IHC of mouse lymphoid tissue. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5632 concentration 5 ug/ml.

Western blot

Western blot
DNMT1 antibody LS-B5632 Western blot of Mouse Lung lysate.

Requested From: United States
Date Requested: 8/30/2015

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