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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DLL4 Antibody (aa71‑120) LS‑C107688
DLL4 antibody LS-C107688 is an unconjugated rabbit polyclonal antibody to DLL4 from human, bovine, guinea pig and other species. Validated for WB.
Catalog
Size
Price
LS-C107688-50
50 µg
Unavailable

Popular DLL4 Products

Anti-Delta-4 Antibody - Immunohistochemistry. Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Antibody
Species: Mouse
Applications: ELISA
Western blot of DLL4 in HUVEC cell lysate using 1) preimmune sera at 1:5000 and 2) LS-C148713 at 3 ug/ml, and 3) mouse embryo body lysate at 6 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Species: Human, Mouse
Applications: Western blot
IHC of paraffin-embedded rectum cancer tissues using DLL4 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC with DLL4 antibody on frozen human tonsil.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Frozen, Western blot

Product Description

DLL4 antibody LS-C107688 is an unconjugated rabbit polyclonal antibody to DLL4 from human, bovine, guinea pig and other species. Validated for WB.
About DLL4
Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate. Q9NR61 NM_019074 NP_061947.1

DLL4 Antibody, Delta-like 4 protein Antibody, Delta like 4 Antibody, Delta-like 4 (Drosophila) Antibody, Delta-like 4 homolog Antibody, Drosophila Delta homolog 4 Antibody, Notch ligand DLL4 Antibody, Delta 4 Antibody, Delta ligand 4 Antibody, Delta-like protein 4 Antibody, Delta4 Antibody, Hdelta2 Antibody, Notch ligand delta-2 Antibody


Specifications

Target
Human DLL4
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Guinea pig (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
DLL4 antibody was raised against synthetic peptide located between aa71-120 of human DLL4 (Q9NR61, NP_061947). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog, Bat, Rabbit, Horse, Pig (92%); Galago, Bovine, Guinea pig (85%).
Blocking Peptide
LS-E17841 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107688. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa71-120
Specificity
Human DLL4
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:2500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

DLL4 antibody LS-C107688 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DLL4 antibody LS-C107688 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DLL4 antibody LS-C107688 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DLL4 antibody LS-C107688 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DLL4 antibody LS-C107688 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DLL4 antibody LS-C107688 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DLL4 antibody LS-C107688 Western blot of 721_B cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DLL4 antibody LS-C107688 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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