Rat DLL1

Sheep

Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NS0-derived rrDLL1 extracellular domain.

Extracellular Domain

Recognize rat DLL1. Species sequence homology: mouse (97%), human (91%), over aa18-537.

• IHC (10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Western Blot, Immunohistochemistry and ELISA. Western Blot: 0.1-0.2 ug/ml Detection limit for recombinant rat DLL1 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. Immunohistochemistry: 10 ug/ml Detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4%PFA+14%picric acid in PBS at 4? C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 min at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS. Direct ELISA: 0.5-1.0 ug/ml Detection limit: 0.3 ng/well.~10% cross-reactivity with rhDLL-1 and >1% cross reactivity with rrNotch-1.

Lyophilized from Sterile Solution PBS, 5% Trehalose

40-50% glycerol, PBS

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00548 NCBI: NM_005618 NP_005609.3