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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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DKK1 Antibody (C‑Terminus) LS‑C81144
DKK1 antibody LS-C81144 is an unconjugated rabbit polyclonal antibody to DKK1 from human, rat, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C81144-100
100 µg
Unavailable

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Western blot of human DKK1 in A549 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW using antibody at 1 ug/ml.
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Species: Rat
Applications: Western blot, ELISA

Product Description

DKK1 antibody LS-C81144 is an unconjugated rabbit polyclonal antibody to DKK1 from human, rat, bat and other species. Validated for WB.
About DKK1
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. O94907 NM_012242 NP_036374.1

DKK1 Antibody, Dickkopf-1 like Antibody, DKK-1 Antibody, Dickkopf-1 Antibody, Dickkopf-related protein 1 Antibody, Dickkopf related protein-1 Antibody, HDkk-1 Antibody, Hdkk1 Antibody, Dickkopf-like protein 1 Antibody, SK Antibody


Specifications

Target
Human DKK1
Host
Rabbit
Reactivity
Human, Monkey, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Horse, Pig, Rabbit, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Mouse, Hamster (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
DKK1 antibody was raised against synthetic peptide from C-Terminus of human DKK1 (O94907, NP_036374). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Platypus, Xenopus (100%); Mouse, Hamster, Opossum (92%); Turkey, Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (85%).
Blocking Peptide
LS-E17376 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81144. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human DKK1
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 2.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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