Target
Human DIAPH1
Synonyms
DIAPH1 | DRF1 | DIA1 | Diaphanous-related formin 1 | Diaphanous-related formin-1 | DIAP1 | Protein diaphanous homolog 1 | DFNA1 | HDIA1 | LFHL1
Reactivity
Human, Mouse, Rat, Bat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QTKTSKAKKDQ, from the internal region of the protein sequence according to NP_005210.3NP_001073280.1.
Specificity
Human DIAPH1. This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141 kDa according to Human NP_005210.3 and 139 kDa according to Mouse NP_031884.1). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee