Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Second mitochondria-derived activator of caspase (SMAC) has been implicated in the activation of apoptosis in response to cell stress. SMAC promotes CASP9 activation by binding to IAPs and removing their inhibitory activity. The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes.
Formalin-fixed, paraffin-embedded tissue section of human plasmacytoma (a mature B cell lymphoma) stained for Smac/DIABLO using LS-C148290 at 1:2000. Intense nuclear staining is observed. Hematoxylin-Eos in counterstain.
Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using LS-C148290 at 1:2000. A, normal colon. B and C, colon carcinoma. Only trace Smac/DIABLO immunoreactivity is seen in normal colonic epithelium (A), which is seen in the apical, foveolar cells. In contrast, strong immunoreactivity is observed in nuclear (B) and cytoplasmic (C) locations in the colon carcinoma specimen. Hematoxylin-Eosin counterstain.
Western blot of Smac/DIABLO human tissues and cell lines using LS-C148290 at 1:2000. Legend. SMAC: full-length recombinant smac. RS1846 and Jurkat are tumor cell lines. All tissues are from normal donors except colon carcinoma (Colon Ca). SMAC expression was detected in all samples except spleen and normal colon.