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| Catalog Number | Size | Price |
|---|---|---|
| LS-B221-0.2 | 0.2 ml | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human DIABLO / SMAC Antibody (clone 78‑1‑118, aa1‑55, IHC, WB) LS‑B221
IHC‑plus™ Monoclonal Mouse anti‑Human DIABLO / SMAC Antibody (clone 78‑1‑118, aa1‑55, IHC, WB) LS‑B221
Note: This antibody replaces LS-C2648
Antibody:
DIABLO / SMAC Mouse anti-Human Monoclonal (aa1-55) (78-1-118) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
DIABLO / SMAC Mouse anti-Human Monoclonal (aa1-55) (78-1-118) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
SMAC antibody LS-B221 is an unconjugated mouse monoclonal antibody to SMAC (DIABLO) (aa1-55) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human DIABLO / SMAC
Synonyms
DIABLO | DFNA64 | DIABLO-S | Diablo homolog, mitochondrial | SMAC3 | Mitochondrial Smac protein | SMAC
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
Clone
78-1-118
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Recombinant mature human Smac/DIABLO (lacks amino acids 1-55 present in Smac/DIABLO precursor).
Epitope
aa1-55
Specificity
Smac/DIABLO.
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:500)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B221 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B221 was determined to be 1:500.
Presentation
0.1% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About DIABLO / SMAC
Validation
Anti-DIABLO / SMAC antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-DIABLO / SMAC antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
See More About...
LSBio Ratings
IHC-plus™ DIABLO / SMAC Antibody (aa1-55, clone 78-1-118) for IHC, WB/Western LS-B221 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Source:
E. coli
Tag:
6His, N-terminus
Reactivity:
Human
Range:
15.6-1000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
