Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of kidney tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-DHFR mouse monoclonal antibody. (Dilution 1:50).
Anti-DHFR mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFR.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-DHFR monoclonal antibody.
Requested From: United States
Date Requested: 2/22/2017