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Catalog Number Size Price
LS-C413162-100 100 µl (1 mg/ml) $321 
DGK-IOTA / DGKI Antibody - Immunofluorescent analysis of DGK iota staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DGK-IOTA / DGKI Antibody - Western blot analysis of DGK iota expression in HEK293T (A); HeLa (B); mouse brain (C) whole cell lysates.
DGK-IOTA / DGKI Antibody - Immunofluorescent analysis of DGK iota staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DGK-IOTA / DGKI Antibody - Western blot analysis of DGK iota expression in HEK293T (A); HeLa (B); mouse brain (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human DGK‑IOTA / DGKI Antibody (C‑Terminus, IHC, IF, WB) LS‑C413162

Polyclonal Rabbit anti‑Human DGK‑IOTA / DGKI Antibody (C‑Terminus, IHC, IF, WB) LS‑C413162

Antibody:
DGK-IOTA / DGKI Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$321
LS-C413162-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DGK-IOTA / DGKI Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
DGKI antibody LS-C413162 is an unconjugated rabbit polyclonal antibody to DGKI (DGK-IOTA) (C-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human DGK-IOTA / DGKI
Synonyms
DGKI | DAG kinase iota | DGK-IOTA | Diacylglycerol kinase iota | Diacylglycerol kinase, iota | Diglyceride kinase iota
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human DGK iota.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of DGK iota protein.
Applications
  • IHC (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DGK-IOTA / DGKI
O75912 NM_004717 NP_004708.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024