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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DGK‑IOTA / DGKI Antibody (aa991‑1040) LS‑C118721
DGKI antibody LS-C118721 is an unconjugated rabbit polyclonal antibody to DGKI (DGK-IOTA) from human and rat. Validated for IF, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C118721-50
50 µl
Unavailable
LS-C118721-100
100 µl
Unavailable

Popular DGK-IOTA / DGKI Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western blot of DGK- (K1023) pAb in extracts from HeLa cells.
Species: Human, Rat
Applications: IHC, Immunofluorescence, Western blot
Western blot of DGK-iota antibody
Species: Human, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
Immunofluorescent analysis of DGK iota staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot

Product Description

DGKI antibody LS-C118721 is an unconjugated rabbit polyclonal antibody to DGKI (DGK-IOTA) from human and rat. Validated for IF, Peptide-ELISA and WB.
About DGK-IOTA / DGKI
DGK-IOTA / DGKI is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. O75912 NM_004717 NP_004708.1

DGKI Antibody, DAG kinase iota Antibody, DGK-IOTA Antibody, Diacylglycerol kinase iota Antibody, Diacylglycerol kinase, iota Antibody, Diglyceride kinase iota Antibody


Specifications

Target
Human DGK-IOTA / DGKI
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Immunogen
DGK-IOTA / DGKI antibody was raised against synthetic peptide from human DGKI (aa991-1040).
Epitope
aa991-1040
Specificity
DGKI Antibody detects endogenous levels of total DGKI protein.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence

Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence

Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence

Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.
Immunofluorescence of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/21/2018

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