Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
IHC - Paraffin
Western blot (1:500 - 1:1000)
Specificity and Use
DFFB antibody was raised against synthetic peptide corresponding to amino acids 205-222 of mouse CAD ( AA 205-222 ).
CAD (Caspase Activated Deoxyribonuclease) is the murine homologue of human DFF-40. CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA. AHP486 recognizes the 40kD CAD in Western blots.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease).