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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DFFA / ICAD / DFF45 Antibody (clone 5C4) LS‑C114353
DFF45 antibody LS-C114353 is an unconjugated mouse monoclonal antibody to human DFF45 (DFFA / ICAD). Validated for IF and WB.
Catalog
Size
Price
LS-C114353-100
100 µl
Unavailable

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Product Description

DFF45 antibody LS-C114353 is an unconjugated mouse monoclonal antibody to human DFF45 (DFFA / ICAD). Validated for IF and WB.
About DFFA / ICAD / DFF45
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kD DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. O00273 NM_004401 NP_004392.1

DFFA Antibody, DFF-45 Antibody, DFF1 Antibody, Dna fragmentation factor-45 Antibody, ICAD Antibody, Inhibitor of CAD Antibody, DFF45 Antibody


Specifications

Target
Human DFFA / ICAD / DFF45
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [5C4]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:2500 - 1:5000)
Immunogen
Full length human recombinant protein of human DFFA (NP_004392) produced in E. coli.
Specificity
Human ICAD / DFF45
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-DFFA mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.
Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.

Immunofluorescence

Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.
Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.

Western blot

DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.
DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.

Immunofluorescence

Anti-DFFA mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.
Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.

Immunofluorescence

Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.
Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.

Western blot

DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.
DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.

Immunofluorescence

Anti-DFFA mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.
Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.

Immunofluorescence

Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.
Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.

Western blot

DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.
DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.

Immunofluorescence

Anti-DFFA mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.
Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA.

Immunofluorescence

Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.
Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.

Western blot

DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.
DFF45 antibody (5C4) at 1:5000 dilution + Hela cell lysate.


Requested From: 
Date Requested: 6/18/2018

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