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Anti-DES / Desmin Antibody (clone 10H7D2) IHC-plus™ LS-B1579

Note: This antibody replaces LS-C41446

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DES / Desmin Antibodies

Anti-DES / Desmin Antibody (C-Terminus) IHC-plus™ LS-B2264
Rabbit Polyclonal to Human DES / Desmin
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-DES / Desmin Antibody (clone D33) LS-C88137
Mouse Monoclonal [clone D33] (IgG1,k) to Human DES / Desmin
Human, Baboon, Monkey, Rat, Bovine, Cat, Dog, Hamster, Chicken
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry Image
Anti-DES / Desmin Antibody (aa454-468) IHC-plus™ LS-B4463
Goat Polyclonal to Human DES / Desmin
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 10H7D2] (IgG1) to Human DES / Desmin
Human
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated

Details

Human DES / Desmin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [10H7D2]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:1000 - 1:2000)
  • IHC - Frozen (1:1000 - 1:2000)
  • Western blot (1:1000 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

DES / Desmin antibody was raised against recombinant human DES / Desmin.
Ni-NTA purified truncated recombinant Desmin expressed in E. Coli strain BL21 (DE3)
Immunohistochemistry: LS-B1579 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1579 was determined to be 1:1000-1:2000.

Packaging

PBS, 0.03% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About DES / Desmin

P17661 NM_001927 NP_001918.3

DES Antibody, ARVC7 Antibody, ARVD7 Antibody, CMD1I Antibody, CSM1 Antibody, CSM2 Antibody, MFM1 Antibody, SCPNK Antibody, Desmin Antibody, Intermediate filament protein Antibody

Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.

Immunohistochemistry

Immunohistochemistry
Anti-DES / Desmin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1579 dilution 1:1000.

Western blot

Western blot
Western blot using Desmin mouse monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Desmin cDNA (2).

Requested From: United States
Date Requested: 12/5/2016

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