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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DEFB3 / Beta Defensin 3 Antibody IHC‑plus™ LS‑B86 Best Seller
Note: This antibody replaces LS-C2782, LS-C35131
Beta Defensin 3 antibody LS-B86 is an unconjugated rabbit polyclonal antibody to Beta Defensin 3 (DEFB3) from human and rat. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B86-100
100 µl (1 mg/ml)
Unavailable

Popular DEFB3 / Beta Defensin 3 Products

Antibody
Species: Mouse, Human
Applications: Western blot, ELISA
Antibody
Species: Mouse, Human
Applications: Western blot, ELISA
Sandwich ELISA of HBD3 / DEFB3 antibody LS-C104293.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, ELISA
Sandwich ELISA of HBD3 / DEFB3 antibody LS-C104293.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, ELISA

Product Description

Beta Defensin 3 antibody LS-B86 is an unconjugated rabbit polyclonal antibody to Beta Defensin 3 (DEFB3) from human and rat. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About DEFB3 / Beta Defensin 3
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. The DEFB103A and DEFB103A genes encode the same protein, defensin beta 103, also known as Beta Defensin 3, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans.

Specifications

Target
Human DEFB3 / Beta Defensin 3
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:10 - 1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide: GIINTLQKYYC, corresponding to amino acids 23/33 of Human beta 3 Defensin BLAST 'GIINTLQKYYC'
Usage
Immunohistochemistry: LS-B86 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B86 was determined to be 10 ug/ml.
Presentation
PBS, 0.02% sodium azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.
Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.

Immunohistochemistry

Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.
Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.

Immunohistochemistry

Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.
Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.

Immunohistochemistry

Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.
Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.

Requested From: 
Date Requested: 7/15/2018

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