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Anti-DEFB3 / Beta Defensin 3 Antibody IHC-plus™ LS-B86

Note: This antibody replaces LS-C2782, LS-C35131

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DEFB3 / Beta Defensin 3 Antibodies

Anti-DEFB3 / Beta Defensin 3 Antibody IHC-plus™ LS-B86
Rabbit Polyclonal (IgG) to Human DEFB3 / Beta Defensin 3
Human
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DEFB3 / Beta Defensin 3 Antibody (N-Terminus) LS-C35132
Rabbit Polyclonal (IgG) to Mouse DEFB3 / Beta Defensin 3
Mouse, Human
Western blot, ELISA
Unconjugated
Anti-DEFB3 / Beta Defensin 3 Antibody (N-Terminus) LS-C35133
Rabbit Polyclonal to Mouse DEFB3 / Beta Defensin 3
Mouse, Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human DEFB3 / Beta Defensin 3
Human
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human DEFB3 / Beta Defensin 3
Rabbit
Human (tested or 100% immunogen sequence identity)
Dog, Pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:10 - 1:500)
  • ICC (1:10 - 1:500)
  • Immunofluorescence (1:10 - 1:500)
  • Western blot (1:500)

Specificity and Use

Synthetic peptide: GIINTLQKYYC, corresponding to amino acids 23/33 of Human beta 3 Defensin BLAST 'GIINTLQKYYC'
Immunohistochemistry: LS-B86 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B86 was determined to be 10 ug/ml.

Packaging

PBS, 0.02% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About DEFB3 / Beta Defensin 3

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. The DEFB103A and DEFB103A genes encode the same protein, defensin beta 103, also known as Beta Defensin 3, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans.

Immunohistochemistry

Immunohistochemistry
Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B86 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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