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Catalog Number Size Price
LS-C313094-10 10 µg $318 
LS-C313094-100 100 µg (0.5 mg/ml) $470 
DDX4 / VASA Antibody - DDX4 / VASA antibody. IHC(F): Rat Intestine Tissue.
DDX4 / VASA Antibody - IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody. DDX4/MVH was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDX4/MVH antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDX4 / VASA Antibody - DDX4 / VASA antibody. IHC(P): Rat Ovary Tissue.
DDX4 / VASA Antibody - DDX4 / VASA antibody. IHC(P): Rat Testis Tissue.
DDX4 / VASA Antibody - DDX4 / VASA antibody. ICC: MCF-7 Cell.
DDX4 / VASA Antibody - Anti-DDX4/MVH antibody, Western blotting Lane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: Mouse Ovary Tissue Lysate
DDX4 / VASA Antibody - Anti-DDX4/MVH antibody, Western blotting Lane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: HELA Cell Lysate
DDX4 / VASA Antibody - WB of DDX4 / VASA antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: Mouse Testis Tissue Lysate. Lane 3: HELA Cell Lysate.
DDX4 / VASA Antibody - Flow Cytometry analysis of PC-3 cells using anti-DDX4/MVH antibody. Overlay histogram showing PC-3 cells stained with anti-DDX4/MVH antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DDX4 / VASA Antibody - Flow Cytometry analysis of SiHa cells using anti-DDX4/MVH antibody. Overlay histogram showing SiHa cells stained with anti-DDX4/MVH antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DDX4 / VASA Antibody - DDX4 / VASA antibody. IHC(F): Rat Intestine Tissue.
DDX4 / VASA Antibody - IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody. DDX4/MVH was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-DDX4/MVH antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
DDX4 / VASA Antibody - DDX4 / VASA antibody. IHC(P): Rat Ovary Tissue.
DDX4 / VASA Antibody - DDX4 / VASA antibody. IHC(P): Rat Testis Tissue.
DDX4 / VASA Antibody - DDX4 / VASA antibody. ICC: MCF-7 Cell.
DDX4 / VASA Antibody - Anti-DDX4/MVH antibody, Western blotting Lane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: Mouse Ovary Tissue Lysate
DDX4 / VASA Antibody - Anti-DDX4/MVH antibody, Western blotting Lane 1: Rat Testis Tissue LysateLane 2: Mouse Testis Tissue LysateLane 3: HELA Cell Lysate
DDX4 / VASA Antibody - WB of DDX4 / VASA antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: Mouse Testis Tissue Lysate. Lane 3: HELA Cell Lysate.
DDX4 / VASA Antibody - Flow Cytometry analysis of PC-3 cells using anti-DDX4/MVH antibody. Overlay histogram showing PC-3 cells stained with anti-DDX4/MVH antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
DDX4 / VASA Antibody - Flow Cytometry analysis of SiHa cells using anti-DDX4/MVH antibody. Overlay histogram showing SiHa cells stained with anti-DDX4/MVH antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human DDX4 / VASA Antibody (aa253‑272, IHC, WB) LS‑C313094

Polyclonal Rabbit anti‑Human DDX4 / VASA Antibody (aa253‑272, IHC, WB) LS‑C313094

Note: This antibody replaces LS-C389368
Antibody:
DDX4 / VASA Rabbit anti-Human Polyclonal (aa253-272) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313094-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DDX4 / VASA Rabbit anti-Human Polyclonal (aa253-272) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
VASA antibody LS-C313094 is an unconjugated rabbit polyclonal antibody to VASA (DDX4) (aa253-272) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human DDX4 / VASA
Synonyms
DDX4 | DEAD box protein 4 | Vasa homolog | VASA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272 aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.
Epitope
aa253-272
Specificity
Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. .
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDX4 / VASA

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/25/2024