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Anti-DDX27 Antibody (aa35-84) LS-C110162

Catalog	Size	Price
LS-C110162-50	50 µg	Unavailable

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26 DDX27 Antibodies

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Anti-DDX27 Antibody (clone 2251C2a) LS-C184388

Antibody:	Mouse Monoclonal [clone 2251C2a] (IgG1) to Human DDX27
Reactivity:	Human, Mouse
Application:	ICC, Western blot, Flow Cytometry, Dot Blot
Format:	Unconjugated

Anti-DDX27 Antibody (aa770-796, AP) LS-C234490

Antibody:	Rabbit Polyclonal (IgG) to Human DDX27
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	AP Conjugated

Anti-DDX27 Antibody (aa770-796, APC) LS-C234492

Antibody:	Rabbit Polyclonal (IgG) to Human DDX27
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	APC Conjugated

Anti-DDX27 Antibody (aa777-796) LS-C320781

Antibody:	Rabbit Polyclonal (IgG) to Human DDX27
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	Unconjugated

Anti-DDX27 Antibody (clone 2251C2a) LS-C342435

Antibody:	Mouse Monoclonal [clone 2251C2a] (IgG1) to Human DDX27
Reactivity:	Human, Mouse
Application:	ICC, Immunofluorescence, Western blot, Flow Cytometry, Dot Blot
Format:	Unconjugated

Antibody:	Rabbit Polyclonal to Human DDX27
Reactivity:	Human, Monkey, Bat, Dog, Guinea pig, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human DDX27
Host:	Rabbit
Reactivity:	Human, Monkey, Bat, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Mouse, Rat, Horse (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

DDX27 antibody was raised against synthetic peptide located between aa35-84 of human DDX27 (Q96GQ7, NP_060365). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Bat, Rabbit, Guinea pig (100%); Mouse, Rat, Horse (92%); Galago, Opossum (85%).

Blocking Peptide:

LS-E13057 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110162. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa35-84

Specificity:

Human DDX27

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About DDX27

Uniprot: Q96GQ7 NCBI: NM_017895 NP_060365.7

Synonyms: DDX27 Antibody, DEAD box protein 27 Antibody, DRS1 Antibody, HSPC259 Antibody, PP3241 Antibody, RNA helicase-like protein Antibody, RHLP Antibody, Rrp3p Antibody, DJ686N3.1 Antibody

Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

(More About DDX27)

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Western blot

DDX27 antibody LS-C110162 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 8/20/2017

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