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DDX27 Antibody (aa35‑84) LS‑C110162
DDX27 antibody LS-C110162 is an unconjugated rabbit polyclonal antibody to DDX27 from human, bat, dog and other species. Validated for WB.
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
ICC, Western blot, Flow Cytometry, Dot Blot
Species:
Human
Applications:
IHC, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, Western blot, Flow Cytometry, ELISA
Species:
Human, Mouse
Applications:
ICC, Immunofluorescence, Western blot, Flow Cytometry, Dot Blot
Product Description
DDX27 antibody LS-C110162 is an unconjugated rabbit polyclonal antibody to DDX27 from human, bat, dog and other species. Validated for WB.
About DDX27
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Q96GQ7
NM_017895
NP_060365.7
DDX27 Antibody, DEAD box protein 27 Antibody, DRS1 Antibody, HSPC259 Antibody, PP3241 Antibody, RNA helicase-like protein Antibody, RHLP Antibody, Rrp3p Antibody, DJ686N3.1 Antibody
Specifications
Target
Human DDX27
Host
Rabbit
Reactivity
Human, Monkey, Bat, Dog, Guinea pig, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
DDX27 antibody was raised against synthetic peptide located between aa35-84 of human DDX27 (Q96GQ7, NP_060365). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Bat, Rabbit, Guinea pig (100%); Mouse, Rat, Horse (92%); Galago, Opossum (85%).
Blocking Peptide
LS-E13057 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110162. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C110162. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human DDX27
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Western blot
DDX27 antibody LS-C110162 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
DDX27 antibody LS-C110162 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
DDX27 antibody LS-C110162 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
DDX27 antibody LS-C110162 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/24/2018
Date Requested: 6/24/2018
