Products

Antibodies
All Antibodies
Primary Antibodies
Secondary Antibodies
IHC-plus Antibodies
Isotype Control Antibodies

ELISA and Assay Kits
All Kits
Assay Kits
Traditional ELISA Kits
Cell-Based ELISA Kits
DNA-Binding ELISA Kits
Phospho-Specific ELISA Kits
ELISA Development Kits
Chemiluminescent CLIA Kits

Proteins
All Proteins
Recombinant Proteins
Native Proteins
Over-expression Lysates
Cell and Tissue Lysates
Bio-active Proteins
Animal-free Proteins
Synthetic Peptides

Biochemicals
All Biochemicals

Immunohistochemistry
Immunohistochemistry Reagents
Comprehensive IHC Reports
Custom IHC Services
TCR Screening Services
IHC-plus Antibodies

Services

Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Have a question? CustomerSupport@LSBio.com

Resources

Purchasing
Product Ordering Terms and Conditions
Holiday Schedule
About the LSBio Guarantee
About Reviews

Reference Material
About LSBio (LifeSpan BioSciences Inc.)
About Our 2-million Specimen Tissue Bank
About Our IHC Validation Process
About Our IHC-plus™ Immunohistochemistry Protocol
Protocols

Secure Logins
Login to Our Secure ShareFile Site
Login to Our IHC Database

Distributors

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone:	866-819-4732 (Toll Free North America) 206-374-1102 (International)

Fax:	866-206-6909 (Toll Free North America) 206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Username or Email:
Password:

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Wish List

View Cart

Anti-DDX27 Antibody (aa35-84) LS-C110162

Catalog	Size	Price
LS-C110162-50	50 µg	Unavailable

Related Products

26 DDX27 Antibodies

Most Popular DDX27 Antibodies

Anti-DDX27 Antibody (clone 2251C2a) LS-C184388

Antibody:	Mouse Monoclonal [clone 2251C2a] (IgG1) to Human DDX27
Reactivity:	Human, Mouse
Application:	ICC, Western blot, Flow Cytometry, Dot Blot
Format:	Unconjugated

Immunofluorescent staining using DDX27 antibody. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-DDX27 antibody.

Anti-DDX27 Antibody (aa770-796, AP) LS-C234490

Antibody:	Rabbit Polyclonal (IgG) to Human DDX27
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	AP Conjugated

Anti-DDX27 Antibody (aa770-796, APC) LS-C234492

Antibody:	Rabbit Polyclonal (IgG) to Human DDX27
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	APC Conjugated

Anti-DDX27 Antibody (aa770-796) LS-C320781

Antibody:	Rabbit Polyclonal (IgG) to Human DDX27
Reactivity:	Human
Application:	IHC, Western blot, Flow Cytometry, ELISA
Format:	Unconjugated

Anti-DDX27 Antibody (clone 2251C2a) LS-C342435

Antibody:	Mouse Monoclonal [clone 2251C2a] (IgG1) to Human DDX27
Reactivity:	Human, Mouse
Application:	ICC, Immunofluorescence, Western blot, Flow Cytometry, Dot Blot
Format:	Unconjugated

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX27 mAb.

Antibody:	Rabbit Polyclonal to Human DDX27
Reactivity:	Human, Monkey, Bat, Dog, Guinea pig, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

DDX27 antibody LS-C110162 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human DDX27
Host:	Rabbit
Reactivity:	Human, Monkey, Bat, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted:	Mouse, Rat, Horse (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

DDX27 antibody was raised against synthetic peptide located between aa35-84 of human DDX27 (Q96GQ7, NP_060365). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Bat, Rabbit, Guinea pig (100%); Mouse, Rat, Horse (92%); Galago, Opossum (85%).

Blocking Peptide:

LS-E13057 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110162. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa35-84

Specificity:

Human DDX27

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About DDX27

Uniprot: Q96GQ7 NCBI: NM_017895 NP_060365.7

Synonyms: DDX27 Antibody, DEAD box protein 27 Antibody, DRS1 Antibody, HSPC259 Antibody, PP3241 Antibody, RNA helicase-like protein Antibody, RHLP Antibody, Rrp3p Antibody, DJ686N3.1 Antibody

Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

(More About DDX27)

Print This Page Email

Technical Help Review

Review This Product

Western blot

DDX27 antibody LS-C110162 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/25/2018

Get Social With Us!

Catalog Number