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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DDIT3 / CHOP Antibody (aa28‑77) LS‑C201241
CHOP antibody LS-C201241 is an unconjugated rabbit polyclonal antibody to CHOP (DDIT3) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C201241-50
50 µg (1 mg/ml)
Unavailable

Popular DDIT3 / CHOP Products

Anti-DDIT3 / GADD153 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3652 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
IHC of paraffin-embedded human skeletal muscle tissue, using CHOP (Ab-30) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged DDIT3 is approximately 0.03 ng/ml as a capture antibody.
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
Anti-DDIT3 / CHOP antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11229 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CHOP antibody LS-C201241 is an unconjugated rabbit polyclonal antibody to CHOP (DDIT3) from human, mouse, rat and other species. Validated for WB.
About DDIT3 / CHOP
Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. P35638 NM_004083 NP_004074.2

DDIT3 Antibody, CHOP10 Antibody, DDIT-3 Antibody, CHOP Antibody, GADD153 Antibody, C/EBP zeta Antibody, C/EBP-homologous protein Antibody, C/EBP-homologous protein 10 Antibody, CHOP-10 Antibody


Specifications

Target
Human DDIT3 / CHOP
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Sheep (tested or 100% immunogen sequence identity)
Predicted
Monkey, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
DDIT3 / CHOP antibody was raised against synthetic peptide located between aa28-77 of human DDIT3 (F8VS99, NP_001181985). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Galago, Bat, Horse, Guinea pig (100%); Gorilla, Gibbon, Marmoset, Elephant, Panda, Rabbit, Pig (92%); Mouse, Rat, Bovine (85%).
Epitope
aa28-77
Specificity
Human DDIT3 / CHOP
Presentation
Lyophilized from PBS, 2% sucrose.
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of Human Stomach Tumor. DDIT3 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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