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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DDC / DOPA Decarboxylase Antibody (aa359‑408) LS‑C107612
DOPA Decarboxylase antibody LS-C107612 is an unconjugated rabbit polyclonal antibody to DOPA Decarboxylase (DDC) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C107612-50
50 µg
Unavailable

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Product Description

DOPA Decarboxylase antibody LS-C107612 is an unconjugated rabbit polyclonal antibody to DOPA Decarboxylase (DDC) from human, mouse, rat and other species. Validated for WB.
About DDC / DOPA Decarboxylase
The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. P20711 NM_000790 NP_000781.1

DDC Antibody, AADC Antibody, DOPA decarboxylase Antibody


Specifications

Target
Human DDC / DOPA Decarboxylase
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa359-408 of human DDC (P20711, NP_000781). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Guinea pig (100%); Monkey, Rabbit, Horse, Opossum (92%); Panda, Dog (85%); Stickleback, Medaka (84%).
Blocking Peptide
LS-E15239 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107612. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa359-408
Specificity
Human DDC
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

DDC antibody LS-C107612 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DDC antibody LS-C107612 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DDC antibody LS-C107612 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DDC antibody LS-C107612 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DDC antibody LS-C107612 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DDC antibody LS-C107612 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DDC antibody LS-C107612 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DDC antibody LS-C107612 Western blot of Fetal Spleen lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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