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Catalog Number	Size	Price
LS-B364-50	50 µl (85 mg/ml)	$515

DDB1 Antibody - Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

DDB1 Antibody - Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.

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IHC‑plus™ Polyclonal Rabbit anti‑Human DDB1 Antibody (aa198‑213, IHC, WB) LS‑B364

Name: IHC-plus™ DDB1 Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B364-50
Price: 515.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

IHC Validated

(5.0) Rating

Submit Review (2) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C18964

Antibody:

DDB1 Rabbit anti-Human Polyclonal (aa198-213) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA, DB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$515

LS-B364-50

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

DDB1 Rabbit anti-Human Polyclonal (aa198-213) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA, DB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

DDB1 antibody LS-B364 is an unconjugated rabbit polyclonal antibody to DDB1 (aa198-213) from human. It is reactive with human and mouse. Validated for DB, ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.

(2) Publication(s)

Target

Human DDB1

Synonyms

See All DDB1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Delipidated and defibrinated followed by sterile filtration

Modifications

Unmodified

Immunogen

DDB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.

Epitope

aa198-213

Specificity

Reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.

Applications

IHC
IHC - Paraffin (1:500)
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:500)
ELISA (1:2000 - 1:10000)
Dot Blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B364 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B364 was determined to be 1:500.

Presentation

Antiserum, 0.01% Sodium Azide

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About DDB1

Uniprot: Q16531 NCBI: NM_001923 NP_001914.3

Validation

Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

See More About...

About LSBio Validation process

LSBio Ratings

IHC-plus™ DDB1 Antibody (aa198-213) for IHC, WB/Western, IP, ELISA LS-B364 has an LSBio Rating of

(5.0) Rating

Publications (4.1)

Laboratory Validation Score (4)

Learn more about The LSBio Ratings Algorithm

Publications (2)

The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription. Shiyanov P, Hayes SA, Donepudi M, Nichols AF, Linn S, Slagle BL, Raychaudhuri P. Molecular and cellular biology. 1999 19:4935-43.

PubMed: 10373543 PMC: PMC84302

Cullin 4A associates with the UV-damaged DNA-binding protein DDB. Shiyanov P, Nag A, Raychaudhuri P. The Journal of biological chemistry. 1999 274:35309-12.

PubMed: 10585395

Submit a Publication for LS-B364