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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3138-50 | 50 µg (0.5 mg/ml) | $485 |
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2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human DDB1 Antibody (IHC, WB) LS‑B3138
IHC‑plus™ Monoclonal Mouse anti‑Human DDB1 Antibody (IHC, WB) LS‑B3138
Note: This antibody replaces LS-C6388
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
DDB1 antibody LS-B3138 is an unconjugated mouse monoclonal antibody to human DDB1. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human DDB1
Synonyms
DDB1 | DDB p127 subunit | DDBA | HBV X-associated protein 1 | UV-DDB1 | XAP-1 | XPE | XPE-binding factor | UV-damaged DNA-binding factor | XPCE | DNA damage-binding protein 1 | DNA damage-binding protein a | UV-DDB 1 | XAP1 | XPE-BF
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human DDB1 protein
Specificity
Specific for the ~130kD human DDB1 protein. Reactivity has been confirmed with human HeLa cells.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1 - 5 µg/ml)
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3138 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3138 was determined to be 5 ug/ml. Immunoprecipitation: 5-10 ug/reaction. Positive control: Human HeLa cells.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About DDB1
Validation
Anti-DDB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ DDB1 Antibody for IHC, WB/Western, IP, ELISA LS-B3138 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
The CRBN, CUL4A and DDB1 Expression Predicts the Response to Immunomodulatory Drugs and Survival of Multiple Myeloma Patients. Joanna Barankiewicz , Anna Szumera-Cieckiewicz, Aleksander Salomon-Perzynski, Paulina Wieszczy, Agata Malenda, Filip Garbicz, Monika Prochorec-Sobieszek, Irena Misiewicz-Krzeminska, Przemyslaw Juszczynski, Ewa Lech-Maranda. Journal of Clinical Medicine. 2021 Jun;10:2683.
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Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA, Dot Blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
