LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-DDB1 Antibody (aa749-1140) LS-C185341

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.81 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DDB1 Antibodies

Anti-DDB1 Antibody (Internal) IHC-plus™ LS-B364
Rabbit Polyclonal to Human DDB1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-DDB1 Antibody (C-Terminus) IHC-plus™ LS-B1692
Rabbit Polyclonal (IgG) to Human DDB1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DDB1 Antibody IHC-plus™ LS-B3138
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human DDB1
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human DDB1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation (1:500 - 1:1000)

Specificity and Use

DDB1 antibody was raised against recombinant fragment corresponding to a region within amino acids 749 and 1140 of DDB1 (SwissProt Q16531).
aa749-1140
Human DDB1
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About DDB1

Q16531 NM_001923 NP_001914.3

DDB1 Antibody, DDB p127 subunit Antibody, DDBA Antibody, HBV X-associated protein 1 Antibody, UV-DDB1 Antibody, XAP-1 Antibody, XPE Antibody, XPE-binding factor Antibody, UV-damaged DNA-binding factor Antibody, XPCE Antibody, DNA damage-binding protein 1 Antibody, DNA damage-binding protein a Antibody, UV-DDB 1 Antibody, XAP1 Antibody, XPE-BF Antibody

Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Cal27 Xenograft using DDB1 antibody at 1:500 dilution.

Immunocytochemistry

Immunocytochemistry
Immunofluorescence of paraformaldehyde-fixed HeLa using DDB1 antibody at 1:200 dilution.

Western blot

Western blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. DDB1 antibody diluted at 1:5000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. DDB1 antibody diluted at 1:1000.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. DDB1 antibody diluted at 1:1000.

Immunoprecipitation

Immunoprecipitation
DDB1 antibody immunoprecipitates DDB1 protein in IP experiments. IP Sample:1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of DDB1 protein by 2.5 ug of DDB1 antibody 7.5% SDS-PAGE The immunoprecipitated DDB1 protein was detected by DDB1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Requested From: United States
Date Requested: 12/3/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number