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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DCX / Doublecortin Antibody (clone 4A3) LS‑C172397
Doublecortin antibody LS-C172397 is an unconjugated mouse monoclonal antibody to human Doublecortin (DCX). Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C172397-100
100 µl (0.73 mg/ml)
Unavailable

Popular DCX / Doublecortin Products

Antibody
Species: Human, Monkey, Mouse, Rat, Drosophila
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
Species: Mouse, Human, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-DCX / Doublecortin antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Rat brain neural cultures stained with DCX / Doublecortin antibody (green), chicken polyclonal antibody to GFAP (red) and DNA (blue). The DCX / Doublecortin antibody antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX / Doublecortin antibody. This DCX antibody is therefore an excellent marker of developing neuronal cells.
Species: Human, Mouse, Rat, Bovine, Pig
Applications: ICC, Immunofluorescence, Western blot

Product Description

Doublecortin antibody LS-C172397 is an unconjugated mouse monoclonal antibody to human Doublecortin (DCX). Validated for Flow, IF and WB.
About DCX / Doublecortin
DCX / Doublecortin is a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. O43602 NM_000555 NP_000546.2

DCX Antibody, DBCN Antibody, Doublecortin Antibody, Doublin Antibody, Doublecortex Antibody, Lis-X Antibody, SCLH Antibody, LISX Antibody, XLIS Antibody, Lissencephalin-X Antibody


Specifications

Target
Human DCX / Doublecortin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4A3]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
Full length human recombinant protein of human DCX (NP_835365) produced in HEK293T cell.
Specificity
Human DCX / Doublecortin
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.
Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.
Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.
Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunofluorescence

Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.
Anti-DCX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCX.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-DCX antibody (Red), compared to a nonspecific negative control antibody (Blue).


Requested From: 
Date Requested: 6/17/2018

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