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Anti-DCX / Doublecortin Antibody (clone 3E1) LS-C204512

Note: This antibody replaces LS-C180199

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DCX / Doublecortin Antibodies

Anti-DCX / Doublecortin Antibody LS-C36204
Rabbit Polyclonal (IgG) to Human DCX / Doublecortin
Human, Monkey, Mouse, Rat, Drosophila
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated
Anti-DCX / Doublecortin Antibody (aa284-297) LS-C155045
Goat Polyclonal to Mouse DCX / Doublecortin
Mouse, Human, Rat
Western blot, ELISA
Unconjugated
Western blot Image
Anti-DCX / Doublecortin Antibody (clone 3E1) LS-C204512
Mouse Monoclonal [clone 3E1] (IgG2a,k) to Human DCX / Doublecortin
Human, Mouse, Rat, Bovine, Pig
ICC, Immunofluorescence, Western blot
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3E1] (IgG2a,k) to Human DCX / Doublecortin
Human, Mouse, Rat, Bovine, Pig
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human DCX / Doublecortin
Mouse
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [3E1]
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)

Specificity and Use

DCX / Doublecortin antibody was raised against recombinant full length human Lis-A isoform of DCX purified from E. coli.
Clone 3E1 is known to react with Fox3/NeuN from human, cow, pig, mouse, rat and other mammals. Since Fox3/NeuN is highly conserved, it is likely that the antibody is effective on other species also.
The antibody solution can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.

Packaging

PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About DCX / Doublecortin

O43602 NM_000555 NP_000546.2

DCX Antibody, DBCN Antibody, Doublecortin Antibody, Doublin Antibody, Doublecortex Antibody, Lis-X Antibody, SCLH Antibody, LISX Antibody, XLIS Antibody, Lissencephalin-X Antibody

DCX / Doublecortin is a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules.

Immunocytochemistry

Immunocytochemistry
Rat brain neural cultures stained with DCX / Doublecortin antibody (green), chicken polyclonal antibody to GFAP (red) and DNA (blue). The DCX / Doublecortin antibody antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX / Doublecortin antibody. This DCX antibody is therefore an excellent marker of developing neuronal cells.

Western blot

Western blot
Blots of crude rat brain extract from a postnatal 3 day animal stained with DCX / Doublecortin antibody. Two bands at ~45 kDa and ~35 kDa show that DCX / Doublecortin antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A.

Requested From: United States
Date Requested: 12/5/2016

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