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Catalog Number Size Price
LS-C139686-100 100 µg (0.5 mg/ml) $503 
DCX / Doublecortin Antibody - DCX / Doublecortin antibody (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
DCX / Doublecortin Antibody - DCX / Doublecortin antibody Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
DCX / Doublecortin Antibody - DCX / Doublecortin antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DCX / Doublecortin Antibody - DCX / Doublecortin antibody (0.01µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DCX / Doublecortin Antibody - DCX antibody (0.01 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DCX / Doublecortin Antibody - DCX / Doublecortin antibody (2µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
DCX / Doublecortin Antibody - DCX / Doublecortin antibody Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
DCX / Doublecortin Antibody - DCX / Doublecortin antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
DCX / Doublecortin Antibody - DCX / Doublecortin antibody (0.01µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
DCX / Doublecortin Antibody - DCX antibody (0.01 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Polyclonal Goat anti‑Human DCX / Doublecortin Antibody (aa232‑242, IHC, IF, WB) LS‑C139686

Polyclonal Goat anti‑Human DCX / Doublecortin Antibody (aa232‑242, IHC, IF, WB) LS‑C139686

Antibody:
DCX / Doublecortin Goat anti-Human Polyclonal (aa232-242) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C139686-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DCX / Doublecortin Goat anti-Human Polyclonal (aa232-242) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Doublecortin antibody LS-C139686 is an unconjugated goat polyclonal antibody to Doublecortin (DCX) (aa232-242) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human DCX / Doublecortin
Synonyms
DCX | DBCN | Doublecortin | Doublin | Doublecortex | Lis-X | SCLH | LISX | XLIS | Lissencephalin-X
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KTSANMKAPQS, from the internal region of the protein sequence according to NP_000546.2NP_835365.1NP_835364.1NP_001182482.1.
Epitope
aa232-242
Specificity
Human DCX. This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Applications
  • IHC
  • Immunofluorescence (5 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kD band observed in Mouse fetal Brain lysates (calculated MW of 49.3kD according to Human NP_000546.2 and 40.6kD according to Human NP_835365.1 and Mouse NP_001103692.1). Recommended concentration: 0.01-0.03 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DCX / Doublecortin
O43602 NM_000555 NP_000546.2

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Requested From: United States
Date Requested: 4/17/2024