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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1825-50 | 50 µg (0.5 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human DCK / Deoxycytidine kinase Antibody (N‑Terminus, IHC, WB) LS‑B1825
IHC‑plus™ Polyclonal Rabbit anti‑Human DCK / Deoxycytidine kinase Antibody (N‑Terminus, IHC, WB) LS‑B1825
Note: This antibody replaces LS-C46820
Antibody:
DCK / Deoxycytidine kinase Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
DCK / Deoxycytidine kinase Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Deoxycytidine kinase antibody LS-B1825 is an unconjugated rabbit polyclonal antibody to human Deoxycytidine kinase (DCK) (N-Terminus). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human DCK / Deoxycytidine kinase
Synonyms
DCK | Deoxynucleoside kinase | Deoxycytidine kinase
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human DCK.
Epitope
N-Terminus
Specificity
synthetic peptide corresponding to N-terminal residues of human DCK (deoxycytidine kinase)
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- Western blot (1 µg/ml)
- ELISA (1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1825 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1825 was determined to be 20 ug/ml.
Presentation
PBS, 0.01% Sodium Azide, 50% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About DCK / Deoxycytidine kinase
Validation
Anti-DCK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-DCK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ DCK / Deoxycytidine kinase Antibody (N-Terminus) for IHC, WB/Western, ELISA LS-B1825 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (4)
Establishment and characterization of a new human gallbladder carcinoma cell line. Sekine S, Shimada Y, Nagata T, Moriyama M, Omura T, Yoshioka I, Hori R, Matsui K, Sawada S, Okumura T, Yoshida T, Tsukada K. Anticancer research. 2012 Aug;32:3211-8.
Clinicopathological significance of deoxycytidine kinase expression in esophageal squamous cell carcinoma. Shimada Y, Okumura T, Sekine S, Moriyama M, Hojo S, Matsui K, Sawada S, Nagata T, Fukuoka J, Tsukada K. Molecular and clinical oncology. 2013 1:716-720. (IHC; Human)
The augmented expression of the cytidine deaminase gene by 5-azacytidine predicts therapeutic efficacy in myelodysplastic syndromes. Murakami Y, Kimura Y, Kawahara A, Mitsuyasu S, Miyake H, Tohyama K, Endo Y, Yoshida N, Imamura Y, Watari K, Ono M, Okamura T, Kuwano M. Oncotarget. 2019 March;10:2270-2281.
RRM1 Expression as a Prognostic Biomarker for Unresectable or Recurrent Biliary Tract Cancer Treated with Gemcitabine plus Cisplatin. Jung Won Chun, Boyoung Lee, Weon Seo Park, Nayoung Han , Eun Kyung Hong , Eun Young Park, Sung Sik Han, Sang-Jae Park, Tae Hyun Kim, Woo Jin Lee, Sang Myung Woo. Journal of Clinical Medicine. 2021 Oct;10:4652.
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Gibbon, Monkey, Mouse, Bat, Bovine, Dog, Pig, Rabbit
Applications:
Western blot
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
