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Anti-DCAF6 / NRIP Antibody (aa800-850) IHC-plus™ LS-B2046

Note: This antibody replaces LS-C19347

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular DCAF6 / NRIP Antibodies

Anti-DCAF6 / NRIP Antibody (aa800-850) IHC-plus™ LS-B2046
Rabbit Polyclonal to Human DCAF6 / NRIP
Human, Mouse, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DCAF6 / NRIP Antibody (aa484-533) LS-C206253
Rabbit Polyclonal to Human DCAF6 / NRIP
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Chicken
Western blot
Unconjugated
Western blot Image
Anti-DCAF6 / NRIP Antibody (aa300-350) IHC-plus™ LS-B12342
Rabbit Polyclonal (IgG) to Human DCAF6 / NRIP
Human, Mouse, Rat, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human DCAF6 / NRIP
Human, Mouse, Rat, Dog
IHC - Paraffin, Western blot
Unconjugated

Details

Human DCAF6 / NRIP
Rabbit
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

DCAF6 / NRIP antibody was raised against a portion of amino acids 800-850 of human NRIP.
aa800-850
The amino acid sequence used is 100% homologous in human (isoforms a and b), mouse, rat and dog, 88% homologous in zebrafish and 82% homologous in xenopus.
Immunohistochemistry: LS-B2046 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2046 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

1
Identification of androgen-responsive microRNAs and androgen-related genes in breast cancer. Nakano K, Miki Y, Hata S, Ebata A, Takagi K, McNamara KM, Sakurai M, Masuda M, Hirakawa H, Ishida T, Suzuki T, Ohuchi N, Sasano H. Anticancer research. 2013 Nov;33:4811-9. [Full Text Article] [PubMed:24222117]

About DCAF6 / NRIP

Q58WW2 NM_018442 NP_060912.2

DCAF6 Antibody, ARCAP Antibody, PC326 Antibody, IQ motif and WD repeats 1 Antibody, RP4-745I14.1 Antibody, IQWD1 Antibody, MSTP055 Antibody, NRIP Antibody

Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.

Immunohistochemistry

Immunohistochemistry
Anti-DCAF6 / NRIP antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2046 concentration 10 ug/ml.

Western blot

Western blot
Western blot of NRIP in human muscle tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse muscle tissue lysate and 4) rat muscle tissue lysate using antibody at 1 ug/ml.

Requested From: United States
Date Requested: 12/3/2016

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