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Antibodies > DBF4 > LS-C353288
 
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Catalog Number Size Price
LS-C353288-100 100 µl (1 mg/ml) $299 
DBF4 Antibody - Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DBF4 Antibody - Immunofluorescent analysis of DBF4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DBF4 Antibody - Western blot analysis of DBF4 expression in Jurkat (A); HepG2 (B); SW480 (C) whole cell lysates.
DBF4 Antibody - Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DBF4 Antibody - Immunofluorescent analysis of DBF4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DBF4 Antibody - Western blot analysis of DBF4 expression in Jurkat (A); HepG2 (B); SW480 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human DBF4 Antibody (N‑Terminus, IHC, IF, WB) LS‑C353288

Polyclonal Rabbit anti‑Human DBF4 Antibody (N‑Terminus, IHC, IF, WB) LS‑C353288

Submit Review LSBio Guarantee
Antibody:
DBF4 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353288-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DBF4 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
DBF4 antibody LS-C353288 is an unconjugated rabbit polyclonal antibody to DBF4 (N-Terminus) from human. It is reactive with human, bovine and pig. Validated for ICC, IF, IHC and WB.
Target
Human DBF4
Synonyms
DBF4 | Activator of S phase kinase | CHIF | DBF4 homolog (S. cerevisiae) | Chiffon homolog A | DBF4A | ZDBF1 | Protein DBF4 homolog A | ASK
Host
Rabbit
Reactivity
Human, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human DBF4.
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of DBF4 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DBF4
Q9UBU7 NM_006716 NP_006707.1

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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