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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DBF4 Antibody (clone 6G9) LS‑C133665 Best Seller
DBF4 antibody LS-C133665 is an unconjugated mouse monoclonal antibody to human DBF4. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C133665-100
100 µg (0.5 mg/ml)
Unavailable

Popular DBF4 Products

Western blot of Dbf4 (I18) pAb in extracts from NIH-3T3 treated with H2O2 100uM 30'.
Species: Human, Mouse
Applications: IHC, Western blot
Immunohistochemical analysis of DBF4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Bovine, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of Dbf4 antibody
Species: Human, Monkey, Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

DBF4 antibody LS-C133665 is an unconjugated mouse monoclonal antibody to human DBF4. Validated for ELISA, IF and WB.
About DBF4
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. Q9UBU7 NM_006716 NP_006707.1

DBF4 Antibody, Activator of S phase kinase Antibody, CHIF Antibody, DBF4 homolog (S. cerevisiae) Antibody, Chiffon homolog A Antibody, DBF4A Antibody, ZDBF1 Antibody, Protein DBF4 homolog A Antibody, ASK Antibody


Specifications

Target
Human DBF4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [6G9]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Immunogen
DBF4 antibody was raised against dBF4 (NP_006707, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human DBF4
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.
Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.
Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.
Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to DBF4 on HeLa cell. [antibody concentration 10 ug/ml].

Western blot

Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.
Western blot of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged DBF4 is approximately 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/20/2018

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