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Catalog Number	Size	Price
LS-B6446-50	50 µg (0.5 mg/ml)	$485

DAP3 Antibody - Anti-DAP3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

DAP3 Antibody - Anti-DAP3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

DAP3 Antibody - Goat Anti-DAP3 Antibody Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

DAP3 Antibody - Death Associated Protein 3 antibody (0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.

DAP3 Antibody - Death Associated Protein 3 antibody immunofluorescence staining of MCF7 cells with 5ug/ml antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000.

DAP3 Antibody - Death Associated Protein 3 antibody staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

DAP3 Antibody - Goat Anti-DAP3 Antibody (0.3µg/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescencence.

DAP3 Antibody - Death Associated Protein 3 antibody (1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.

DAP3 Antibody - DAP3 antibody staining (3 ug/ml) of HeLa lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

DAP3 Antibody - Death Associated Protein 3 antibody (0.3µg/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.

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IHC‑plus™ Polyclonal Goat anti‑Human DAP3 Antibody (aa387‑398, IHC, IF, WB) LS‑B6446

Name: IHC-plus™ DAP3 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B6446-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C34641

Antibody:

DAP3 Goat anti-Human Polyclonal (aa387-398) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey

Format:

Unconjugated, Unmodified

$485

LS-B6446-50

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

DAP3 Goat anti-Human Polyclonal (aa387-398) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey

Format:

Unconjugated, Unmodified

Specifications

Description

DAP3 antibody LS-B6446 is an unconjugated goat polyclonal antibody to DAP3 (aa387-398) from human. It is reactive with human and monkey. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human DAP3

Synonyms

See All DAP3 Antibodies

Host

Goat

Reactivity

Human, Monkey (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence NPSLLERHCAYL, from the C-Terminus of protein sequence according to NP_387506.1NP_001186779.1NP_001186780.1.

Epitope

aa387-398

Specificity

Human DAP3. This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).

Applications

IHC
IHC - Paraffin (2.5 - 3.75 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot (0.1 - 1 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:64000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 40 kDa band observed in lysates of cell line HeLa (calculated MW of 45.6 kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About DAP3

Uniprot: P51398 NCBI: NM_033657 NP_387506.1

Validation

Anti-DAP3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Anti-DAP3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

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About LSBio Validation process