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Anti-DAP-5 / EIF4G2 Antibody (aa796-812, clone 39C534.1) IHC-plus™ LS-B2026

Note: This antibody replaces LS-C518, LS-C64500
Catalog Size Price
LS-B2026-50 50 µg (0.5 mg/ml) Unavailable

Related Products

24 DAP-5 / EIF4G2 Antibodies

Most Popular DAP-5 / EIF4G2 Antibodies

Anti-DAP-5 / EIF4G2 Antibody (aa766-815) IHC-plus™ LS-B944
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B944 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-DAP-5 / EIF4G2 Antibody (aa796-812, clone 39C534.1) IHC-plus™ LS-B2026
Mouse Monoclonal [clone 39C534.1] (IgG1) to Human DAP-5 / EIF4G2
Human, Mouse
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-EIF4G2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2026 concentration 10 ug/ml.
Anti-DAP-5 / EIF4G2 Antibody (clone 3B5) LS-C133164
Mouse Monoclonal [clone 3B5] (IgG1,k) to Human DAP-5 / EIF4G2
Human, Rat
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunoperoxidase of monoclonal antibody to EIF4G2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml].
Anti-DAP-5 / EIF4G2 Antibody (N-Terminus) LS-C352147
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Human, Monkey, Mouse, Rat, Bovine, Rabbit
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of EIF4G2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-DAP-5 / EIF4G2 Antibody (aa10-90) LS-C383071
Rabbit Polyclonal (IgG) to Human DAP-5 / EIF4G2
Human, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of DAP-5 antibody

100% Guaranteed
Mouse Monoclonal [clone 39C534.1] (IgG1) to Human DAP-5 / EIF4G2
Human, Mouse
IHC, IHC - Paraffin, Western blot
Unconjugated

Details

Human DAP-5 / EIF4G2
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [39C534.1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

DAP-5 / EIF4G2 antibody was raised against two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.
aa796-812
In Jurkat, a 97 kD band is observed.
Immunohistochemistry: LS-B2026 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2026 was determined to be 10 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.

About DAP-5 / EIF4G2

NM_001418

EIF4G2 Antibody, AAG1 Antibody, Aging-associated protein 1 Antibody, DAP-5 Antibody, DAP5 Antibody, Death-associated protein 5 Antibody, EIF-4G 2 Antibody, EIF-4-gamma 2 Antibody, EIF4G 2 Antibody, Translation repressor nat1 Antibody, p97 Antibody

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus.

Immunohistochemistry

Anti-EIF4G2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2026 concentration 10 ug/ml.
Anti-EIF4G2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2026 concentration 10 ug/ml.

Western blot

Western blot of 15 ug of total cell lysate from Jurkat cells with antibody at 1 ug/ml.
Western blot of 15 ug of total cell lysate from Jurkat cells with antibody at 1 ug/ml.

Requested From: 
Date Requested: 5/24/2018

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