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Anti-DAP-5 / EIF4G2 Antibody (aa766-815) IHC-plus™ LS-B944

Note: This antibody replaces LS-C30371

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DAP-5 / EIF4G2 Antibodies

Anti-DAP-5 / EIF4G2 Antibody (aa766-815) IHC-plus™ LS-B944
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DAP-5 / EIF4G2 Antibody (aa796-812, clone 39C534.1) IHC-plus™ LS-B2026
Mouse Monoclonal [clone 39C534.1] (IgG1) to Human DAP-5 / EIF4G2
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DAP-5 / EIF4G2 Antibody IHC-plus™ LS-B4208
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human DAP-5 / EIF4G2
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human DAP-5 / EIF4G2
Rabbit
Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Rat, Guinea pig, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

DAP-5 / EIF4G2 antibody was raised against synthetic peptide located between aa766-815 of human EIF4G2 (P78344). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%); Mouse, Rat, Guinea pig, Turkey, Chicken (92%).
LS-E14710 - Lyophilized - 100 µg - $145.00
aa766-815
Human EIF4G2
Immunohistochemistry: LS-B944 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B944 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About DAP-5 / EIF4G2

NM_001418

EIF4G2 Antibody, AAG1 Antibody, Aging-associated protein 1 Antibody, DAP-5 Antibody, DAP5 Antibody, Death-associated protein 5 Antibody, EIF-4G 2 Antibody, EIF-4-gamma 2 Antibody, EIF4G 2 Antibody, Translation repressor nat1 Antibody, p97 Antibody

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus.

Immunohistochemistry

Immunohistochemistry
Anti-EIF4G2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B944 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-EIF4G2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B944 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
EIF4G2 antibody LS-B944 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/2/2016

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