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Anti-DAD1 Antibody (C-Terminus) IHC-plus™ LS-B555

Note: This antibody replaces LS-C432, LS-C57097, LS-C43115

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DAD1 Antibodies

Anti-DAD1 Antibody (C-Terminus) IHC-plus™ LS-B555
Rabbit Polyclonal to Human DAD1
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DAD1 Antibody (aa64-92, AP) LS-C221987
Rabbit Polyclonal (IgG) to Human DAD1
Human
Western blot, ELISA
AP Conjugated
Anti-DAD1 Antibody (aa64-92, PE) LS-C221989
Rabbit Polyclonal (IgG) to Human DAD1
Human
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human DAD1
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human DAD1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Western blot (0.5 - 2 µg/ml)

Specificity and Use

DAD1 antibody was raised against synthetic peptide from human DAD1.
LS-E30067 - Liquid - 50 µg - $145.00
C-Terminus
peptide corresponding to 14 amino acids near the C-terminus of human DAD1
Immunohistochemistry: LS-B555 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B555 was determined to be 2.5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About DAD1

P61803 NM_001344 NP_001335.1

DAD1 Antibody, DAD-1 Antibody, Defender against cell death 1 Antibody, OST2 Antibody

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death.

Immunohistochemistry

Immunohistochemistry
Anti-DAD1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B555 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-DAD1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B555 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 ug/ml.

Western blot

Western blot
Western blot of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
 

Requested From: United States
Date Requested: 12/2/2016

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