Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone C-46] (IgG1) to Human Cytokeratin 7+17
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Human Cytokeratin 7+17
Human, Bovine (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [C-46]
Protein A purified
IHC - Paraffin
Western blot (2 - 3 µg/ml)
Immunoprecipitation (2 - 3 µg/ml)
Specificity and Use
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Reacts with Cytokeratin peptides 7 and 17 (54 and 46 kD). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Western Blotting: Recommended dilution - 1 ug/ml. Positive control - HeLa human cell line. Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication. 5 min. in hot Laemmli buffer (reducing conditions.). Immunohistochemistry (paraffin s
PBS, 15 mM sodium azide, approx., pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.