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Catalog Number	Size	Price
LS-C391394-20	20 µg (0.2 mg/ml)	$371
LS-C391394-100	100 µg (0.2 mg/ml)	$574

Cytokeratin 5+8 Antibody - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (SPM268). This image was taken for the unmodified form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human Cytokeratin 5+8 Antibody (clone SPM268, IHC, IF, WB) LS‑C391394

Name: Anti-Cytokeratin 5+8 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C391394-20
Price: 371.0000 USD
Availability: InStock

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Antibody:

Cytokeratin 5+8 Mouse anti-Human Monoclonal (SPM268) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, BSA-free

$371

LS-C391394-20

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

Cytokeratin 5+8 Mouse anti-Human Monoclonal (SPM268) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep

Format:

Unconjugated, Unmodified

Other formats:

Azide-free, BSA-free

Specifications

Description

Cytokeratin 5+8 antibody LS-C391394 is an unconjugated mouse monoclonal antibody to Cytokeratin 5+8 from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.

Target

Human Cytokeratin 5+8

See All Cytokeratin 5+8 Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)

Non-Reactivity

Chicken, Xenopus

Clonality

IgG1,k Monoclonal

Clone

SPM268

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified. Also available Azide-free, BSA-free.

Immunogen

Cytoskeletal preparation from HeLa cells.

Specificity

Human Cytokeratin 5+8

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Immunofluorescence (0.5 - 1 µg/ml)
Western blot (0.5 - 1 µg/ml)
Flow Cytometry (0.5 - 1 µg/10E6 cells)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT.

Presentation

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Storage

Store at 2°C to 8°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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