Human Cytokeratin 5+8

Mouse

Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)

Chicken, Xenopus

IgG1,k Monoclonal

C-50

Unconjugated

Protein G purified

Ready-to-Use. Also available Unmodified or Azide-free, BSA-free.

Cytoskeletal preparation from HeLa cells.

Human Cytokeratin 5+8

• IHC
• IHC - Paraffin (1:1)
• Immunofluorescence
• Western blot
• Flow Cytometry

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Optimal dilution of the Cytokeratin 5 + 8 antibody should be determined by the researcher. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.