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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cytokeratin 5+8 Antibody (clone C‑50) LS‑C391379
Cytokeratin 5+8 antibody LS-C391379 is an unconjugated mouse monoclonal antibody to Cytokeratin 5+8 from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C391379-20
20 µg (0.2 mg/ml)
Unavailable
LS-C391379-100
100 µg
Unavailable

Popular Cytokeratin 5+8 Products

Immunohistochemistry on frozen sections of human liver hepatocytes and bile ducts
Species: Human, Mouse, Rat, Dog, Hamster, Pig, Rabbit
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Antibody
Species: Human, Mouse, Bovine, Pig, Amphibian
Applications: IHC, ELISA
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (SPM268).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (SPM268).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

Cytokeratin 5+8 antibody LS-C391379 is an unconjugated mouse monoclonal antibody to Cytokeratin 5+8 from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
About Cytokeratin 5+8
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specifications

Target
Human Cytokeratin 5+8
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)
Non-Reactivity
Chicken, Xenopus
Clonality
IgG1,k Monoclonal [C-50]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Ready-to-Use, Azide-free.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Cytokeratin 5+8 antibody was raised against cytoskeletal preparation from HeLa cells.
Specificity
Human Cytokeratin 5+8
Usage
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Optimal dilution of the Cytokeratin 5 + 8 antibody should be determined by the researcher. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT.
Presentation
0.01% BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.
Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.
Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.
Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.
Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.

Requested From: 
Date Requested: 7/20/2018

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