• Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-Cytokeratin 5+8 Antibody (clone C-50) LS-C391379

Catalog Size Price
LS-C391379-20 20 µg (0.2 mg/ml) Unavailable
LS-C391379-100 100 µg Unavailable

Most Popular Cytokeratin 5+8 Antibodies

Anti-Cytokeratin 5+8 Antibody (clone RCK102) IHC-plus™ LS-B7162
Mouse Monoclonal [clone RCK102] (IgG1) to Human Cytokeratin 5+8
Human, Mouse, Rat, Dog, Hamster, Pig, Rabbit
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Immunohistochemistry Image
Anti-Cytokeratin 5+8 Antibody (clone C22, Biotin) LS-C193866
Mouse Monoclonal [clone C22] (IgG1) to Human Cytokeratin 5+8
Human, Mouse, Bovine, Pig, Amphibian
Biotin Conjugated
Anti-Cytokeratin 5+8 Antibody (APC) LS-C516314
Mouse Monoclonal (IgG1) to Human Cytokeratin 5+8
Human, Mouse, Rat, Dog, Hamster, Pig, Rabbit
IHC, ICC, Western blot, Flow Cytometry
APC Conjugated
Anti-Cytokeratin 5+8 Antibody (Biotin) LS-C525402
Mouse Monoclonal (IgG1) to Human Cytokeratin 5+8
Human, Mouse, Rat, Dog, Hamster, Pig, Rabbit
IHC, ICC, Western blot, Flow Cytometry
Biotin Conjugated
Anti-Cytokeratin 5+8 Antibody (FITC) LS-C535419
Mouse Monoclonal (IgG1) to Human Cytokeratin 5+8
Human, Mouse, Rat, Dog, Hamster, Pig, Rabbit
IHC, ICC, Western blot, Flow Cytometry
FITC Conjugated

100% Guaranteed
Mouse Monoclonal [clone C-50] (IgG1,k) to Human Cytokeratin 5+8
Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry (applications tested for the base form of this product only)


Human Cytokeratin 5+8
Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)
Chicken, Xenopus
IgG1,k Monoclonal [C-50]
Protein G purified
Unmodified. Also available Ready-to-Use, Azide-free.


  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)

Specificity and Use

Cytokeratin 5+8 antibody was raised against cytoskeletal preparation from HeLa cells.
Human Cytokeratin 5+8
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Optimal dilution of the Cytokeratin 5 + 8 antibody should be determined by the researcher. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at RT.


0.01% BSA, 0.05% sodium azide
2°C to 8°C
For research use only.

About Cytokeratin 5+8

Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. Cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.


Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5 + 8 antibody (C50). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot
Western blot testing of HeLa cell lysate and Cytokeratin 5 + 8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.

Requested From: 
Date Requested: 1/19/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number