Human Cytokeratin 5+8

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein A purified

Unmodified

Human cytokeratin 8, purified from SDS PAGE.

aa353-367

Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot

Suitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material) and Western Blot (Western). Immunohistochemistry: 1:10; Incubation Time 1h at RT, extended with paraffin sections (overnight at 2-8?C). Pretreatment with paraffin embedded sections, protease pretreatment is required prior to antibody application.

Lyophilized from PBS, pH 7.4, 0.09% Sodium Azide, 0.5% BSA

Sterile 40-50% glycerol.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.