Human Cytokeratin 10+13

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

SPM262

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Cytoskeletal preparation extracted from human ectocervical epithelium.

Human Cytokeratin 10+13

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (0.25 - 0.5 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia. it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Optimal dilution of the Cytokeratin 10 + 13 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.