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Anti-Cytochrome B245 Heavy Chain Antibody (clone NL7) LS-C188532

Catalog Size Price
LS-C188532-100 100 µg (1 mg/ml) Unavailable

Most Popular Cytochrome B245 Heavy Chain Antibodies

Anti-Cytochrome B245 Heavy Chain Antibody (clone NL7) LS-C188532
Mouse Monoclonal [clone NL7] (IgG1) to Human Cytochrome B245 Heavy Chain
Human
IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Flow Cytometry Image
Anti-Cytochrome B245 Heavy Chain Antibody (clone CS9) LS-C188534
Mouse Monoclonal [clone CS9] (IgG1) to Human Cytochrome B245 Heavy Chain
Human
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone NL7] (IgG1) to Human Cytochrome B245 Heavy Chain
Human
IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human Cytochrome B245 Heavy Chain
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [NL7]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Frozen
  • Western blot
  • Flow Cytometry (1:25 - 1:200)
  • ELISA

Specificity and Use

Cytochrome B245 solubilized in octyl-beta-glucopyranoside.
Recognizes the heavy chain of cytochrome B245, also known as gp91phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterized by severe recurrent infections. The heavy chain (gp91phox), along with the light chain (p22phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils. It recognizes the native as well as the denatured form of the protein. Clone NL7 has an inhibitory effect on NADPH oxidase activation. Research indicates that this inhibitory effect is exerted during the initial stages of oxidase activation. The antibody does not interfere with the translocation of the cytosolic subunits of NADPH oxidase to the cell membrane.

Packaging

PBS, 0.09% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Cytochrome B245 Heavy Chain


Flow Cytometry

Flow Cytometry
Flow cytometry of human peripheral blood granulocytes with Mouse anti-Human Cytochrome b-245 Heavy Chain . Permeabilised with Leucoperm

Requested From: 
Date Requested: 9/21/2017

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