Human Cytochrome B245 Heavy Chain

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

CS9

Unconjugated

Protein G purified

Unmodified

Cytochrome B245 solubilized in octyl-beta-gluycopyranoside.

Recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterized by severe recurrent infections. The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Clone CS9 also has an inhibitory effect on NADPH oxidase activation.

• IHC - Frozen
• Western blot
• Immunoprecipitation
• Flow Cytometry (1:10 - 1:100)
• ELISA

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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