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Anti-CYP8B1 Antibody (aa471-501) LS-C167247

Catalog Size Price
LS-C167247-400 400 µl Unavailable

Related Products

14 CYP8B1 Antibodies

Most Popular CYP8B1 Antibodies

Anti-CYP8B1 Antibody (aa471-501) LS-C167247
Rabbit Polyclonal to Human CYP8B1
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Anti-CYP8B1 Antibody (aa416-461) LS-C313999
Rabbit Polyclonal to Human CYP8B1
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Anti-CYP8B1 Antibody (aa357-406) IHC-plus™ LS-B12483
Rabbit Polyclonal (IgG) to Human CYP8B1
Human, Mouse
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-CYP8B1 Antibody (aa340-420) LS-C383031
Rabbit Polyclonal (IgG) to Human CYP8B1
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Western blot of CYP8B1 antibody
Anti-CYP8B1 Antibody LS-C675073
Rabbit Polyclonal (IgG) to Human CYP8B1
Human
Unconjugated
Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

100% Guaranteed
Rabbit Polyclonal to Human CYP8B1
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human CYP8B1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E9780 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C167247. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa471-501
This CYP8B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-501 amino acids from the C-terminal region of human CYP8B1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CYP8B1

Q9UNU6 NM_004391 NP_004382.2

CYP8B1 Antibody, CYP12 Antibody, Cytochrome P450 8B1 Antibody, CP8B Antibody, Sterol 12-alpha-hydroxylase Antibody, Sterol 12alpha-hydroxylase Antibody, CYPVIIIB1 Antibody

CYP8B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CYP8B1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of CYP8B1 Antibody in K562 cell line lysates (35 ug/lane). CYP8B1 (arrow) was detected using the purified antibody.
Western blot of CYP8B1 Antibody in K562 cell line lysates (35 ug/lane). CYP8B1 (arrow) was detected using the purified antibody.

Flow Cytometry

CYP8B1 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CYP8B1 Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 5/22/2018

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