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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYP3A4 / Cytochrome P450 3A4 Antibody (aa 243‑430, clone 3H8) LS‑C169171
Cytochrome P450 3A4 antibody LS-C169171 is an unconjugated mouse monoclonal antibody to human Cytochrome P450 3A4 (CYP3A4). Validated for ELISA, Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C169171-100
100 µg (1 mg/ml)
Unavailable

Popular CYP3A4 / Cytochrome P450 3A4 Products

Antibody
Species: Human
Applications: Western blot, Functional Assay
Western blot of cytochrome P450 (3A4 isoforms) on rat liver extract. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Rat
Applications: Western blot
CYP3A4 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

Cytochrome P450 3A4 antibody LS-C169171 is an unconjugated mouse monoclonal antibody to human Cytochrome P450 3A4 (CYP3A4). Validated for ELISA, Flow, IF, IHC and WB.
About CYP3A4 / Cytochrome P450 3A4
CYP3A4 / Cytochrome P450 3A4 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. P08684 D00003 BAA00001.1

CYP3A4 Antibody, Albendazole monooxygenase Antibody, Albendazole sulfoxidase Antibody, Cyp3a3 Antibody, CYPIIIA3 Antibody, CYPIIIA4 Antibody, Cytochrome P450 3A3 Antibody, Cytochrome P450 3A4 Antibody, CP33 Antibody, CP34 Antibody, CYP3A Antibody, Cytochrome P450 NF-25 Antibody, HLP Antibody, Nifedipine oxidase Antibody, Quinine 3-monooxygenase Antibody, NF-25 Antibody, p450C3 Antibody, Cytochrome P450 HLp Antibody, Cytochrome P450-PCN1 Antibody, Glucocorticoid-inducible P450 Antibody, p450-III, steroid inducible Antibody, p450PCN1 Antibody


Specifications

Target
Human CYP3A4 / Cytochrome P450 3A4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3H8]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human CYP3A4 expressed in E. Coli.\
Epitope
aa 243-430
Specificity
Human
Presentation
Purified antibody in PBS containing 0.05% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)

Western blot

Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)

Western blot

Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)

Western blot

Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded liver cancer tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded human brain tissues using CYP3A4 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CYP3A4 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)
Western blot using CYP3A4 monoclonal antibody against human CYP3A4 (AA: 243-430) recombinant protein. (Expected MW is 47.5 kDa)

Western blot

Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CYP3A4 monoclonal antibody against HEK293 (1) and CYP3A4 (AA: 243-430)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/24/2018

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